| Idteknologi | 670 |
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| Judul | Aktivitas Aktinomisetes Dari Bangka-BelitungKoleksi Bidang Mikrobiologi, Puslit Biologi- Lipi Dalam? |
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| Abstrak | chitinase, clear zone, spectrophotometer |
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| Katakunci | Teknologi Lingkungan |
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| Kategori | 7 |
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| Mediamasa | http://www.kelair.bppt.go.id/Jtl/2010/vol11-3/11enzim.pdf |
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| Alamatlink | Yati Sudaryati Soeka, Evi Triana dan Ninu Setianingrum |
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| Penulis | ceuceu_lipi@yahoo.com |
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| Instansi | The aim of the research was to know the capability of actinomycetes isolate from Bangka Belitung, which stored at Microbiology-LIPI Culture Collection, in producing chitinase enzyme. This isolate which could produce chitinolitic enzyme, signed by clear zone at medium contain 1% chitine. The chitinase activity of the isolate which incubated for 1-7 days in the room temperature was analyzed by spectrophotometer in ? 584 nm. The result of this experiment was highest chitinase activities with incubated for 3 days, were 1.66 . 10-2 U/ml. Maximum chitinase activities was found at 1% starch soluble substrate 2.83 . 10-2, pH 8.0 and at 50?C condition were 9.3 . 10-2 and 12.98 . 10-2 U/ml respectively. |
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